Does weight lifting reduce vitamin D? in General Board Posted Monday at 09:09 PM Hey tmac, I don't think that working out would deplete your D3, but I am hardly an expert! In addition, you are taking a lot of it daily. When was your last blood work to check your level of D3? I definitely recommend that you have a blood draw to check your D at a minimum. Exercise helps some with their hits and makes them worse for others. Just the luck of the draw I think. But, it sounds like this is a recent change for you. So, I stand by, get a blood test and see where your D currently stands. You can order one from Lab Corp in many states yourself and it is pretty cheap. ATB!