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Hey Cast Iron, I suspect the lab assay method being used to measure your 25(OH)D3 has an upper measurement limit around 400 nmol/L (160 ng/mL). You need to ask your PCP to specify the LC-MS/MS: liquid chromatography-dual mass spectrometry assay for your 25(OH)D3. This assay method has a maximum measurement of 512 ng/mL for the combined D2 - D3. As your 25(OH)D2 measurement is likely less than 4 ng/mL, this assay method should measure your 25(OH)D3 as high as 508 ng/mL. If you followed the treatment protocol I had published on the VitaminDWiki web site at the following link, you would have seen the recommended assay be done at Quest Diagnostics with the QuestAssureD™ 25-Hydroxyvitamin D (D2, D3). http://www.vitamindwiki.com/tiki-download_wiki_attachment.php?attId=7708 The following chart of my assays for 25(OH)D3 were measured with the Quest Diagnostics QuestAssureD™ 25-Hydroxyvitamin D (D2, D3) assay method. As you can see, when I stopped taking vitamin D3 for three months in a 25(OH)D3 burn down test, my 25(OH)D3 assay dropped by 45 ng/mL (112.5 nmol/L) or 15 ng/mL per month. I might add that I remained CH pain free the entire time. How is your head? Are you still CH pain free? Take care and please keep us posted, V/R Batch